Assessment of normal erythropoiesis by flow cytometry: important considerations for specimen preparation

摘要: Summary Introduction The extension of quantitative flow cytometric studies to the erythroid lineage in patients with suspected myelodysplastic syndrome has prompted a reassessment cell surface antigen expression during normal erythropoiesis. Erythropoiesis and pathologic bone marrows was studied determine expected antigenic relationships maturing cells. Methods A total 200 marrow specimens were evaluated by multidimensional cytometry (MDF). Samples prepared using either NH4Cl lysis or Ficoll density gradient separation. Results Normal development is described as two-step process observable intensity between CD235a, CD71, CD45, CD105, CD34, CD117, CD36. The variability these intensities (CV) determined. A comparison processing techniques determined optimal analytic technique for analysis early-stage cells. Nucleic acid staining DRAQ5 revealed that allows reticulocytes mature erythrocytes otherwise eliminated lysis. Conclusion These data demonstrate while alters light scatter characteristics precursors, it did not alter nucleic content. defined. These provide basis individuals those dysplasia associated syndromes.