Characterization of a diagnostic Fab fragment binding trimeric Lewis X
作者： Daniël C de Geus , Anne‐Marie M van Roon , Ellen AJ Thomassen , Cornelis H Hokke , André M Deelder
DOI: 10.1002/PROT.22356
关键词:
摘要: Lewis X trisaccharides normally function as essential cell–cell interaction mediators. However, oligomers of expressed by the parasite Schistosoma mansoni seem to be related its evasion immune response human host. Here we show that monoclonal antibody 54-5C10-A, which is used diagnose schistosomiasis in humans, interacts with at least three trisaccharides, but not monomeric X. We describe sequence and 2.5 A crystal structure Fab fragment infer a possible mode binding polymeric from docking studies. Our studies indicate radically different compared 291-2G3-A, specific for X, thus providing structural explanation diagnostic success 54-5C10-A. Proteins 2009. © 2008 Wiley-Liss, Inc.
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