Intrinsic Bias and Lineage Restriction in the Phenotype Determination of Dopamine and Neuropeptide Y Amacrine Cells

摘要: Blastomere lineages are differentially biased to produce different neurotransmitter subtypes of amacrine cells ([Huang and Moody, 1995][1],[1997][2]). To elucidate when this bias is acquired, we examined at early developmental times. Our experiments demonstrate that the express dopamine neuropeptide Y fates involves several steps before formation definitive optic cup. At cleavage stages, a retinal progenitor contributes large numbers already its normal repertoire cells, as revealed by transplantation new location, whereas fate fewer modified position. neural plate not all progenitors multipotent. Nearly one-half populate only inner nuclear layer enriched in cells. During vesicle an appropriate mitotic tree required for Y, but serotonin, cell clusters form. Thus, acquisition intrinsic maternal factors cleavage, restriction plate, specified patterns vesicle. each these subset embryonic contributing biased; remaining maintain multipotency. from earliest retina dynamic mosaic. This first experimental demonstration decisions occur during periods advance events described later, committed retina. [1]: #ref-19 [2]: #ref-20