The perplexed and confused mutations affect distinct stages during the transition from proliferating to post-mitotic cells within the zebrafish retina.

摘要: To identify and study genes essential for vertebrate retinal development, we are screening zebrafish embryos mutations that disrupt histogenesis. Key steps in retinogenesis include withdrawal from mitosis by multipotent neuroepithelial cells, specification to particular cell types, migration the appropriate laminar positions, molecular morphological differentiation. In this study, have identified two recessive affect transition of proliferating cells postmitotic cells. Both perplexed confused mutant phenotypes were initially detectable when first began leave cycle. At time, each retina showed increased death a lack Cell was found be apoptotic both retinas based on TUNEL analysis activation caspase-3. TUNEL-phosphoRb-BrdU colocalization studies indicated mutation caused transitioning proliferative state. For mutation, revealed only subset induced activate apoptosis. Mosaic demonstrated within functions noncell-autonomously. Furthermore, whole lens or eye cup transplantations defect intrinsic retina. with acts cell-autonomously. From these studies, conclude at distinct stages during