Ex vivo bioluminescence imaging of late gestation ewes following intrauterine inoculation with lux-modified Escherichia coli

摘要: Abstract Our objectives were to develop an ovine model for Escherichia coli -induced preterm delivery, and monitor E. (lux modified photonic detection) invasion of the fetal environment—ewes (124 ± 18 d gestation) received intrauterine inoculations using -lux as follows: control ( n  = 5), 1.2 × 10 6  CFU/ml 5.6 × 10  = 5) -lux. Preterm delivery occurred between 48 120 h post-inoculation in 60%, 60% ewes infected with 1.2, -lux, respectively, presence emitting bacteria confirmed by real-time imaging lamb tissues. In summary, and/or distress observed a majority inoculated ewes. Finally, use was feasible means bacterial ex vivo .