Development of a chinchilla model to allow direct, continuous, biophotonic imaging of bioluminescent nontypeable Haemophilus influenzae during experimental otitis media.
作者： Laura A. Novotny , Kevin M. Mason , Lauren O. Bakaletz
DOI: 10.1128/IAI.73.1.609-611.2005
关键词: Bioluminescence 、 Bioreporter 、 Microbiology 、 Pasteurellaceae 、 Bacterial outer membrane 、 Operon 、 Biology 、 Otitis 、 Chinchilla 、 Haemophilus influenzae
摘要: We transformed a nontypeable Haemophilus influenzae clinical isolate with plasmid containing the luxCDABE operon driven by H. outer membrane protein P2 promoter. Herein, we demonstrate ability to detect bioluminescence and monitor infection within nasopharynges, eustachian tubes, middle ears of chinchillas after intranasal transbullar challenges.
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Michael K Winson, Simon Swift, Leigh Fish, John P Throup, Frieda Jørgensen, Siri Ram Chhabra, Barrie W Bycroft, Paul Williams, Gordon S.A.B Stewart, Construction and analysis of luxCDABE-based plasmid sensors for investigating N-acyl homoserine lactone-mediated quorum sensing Fems Microbiology Letters. ,vol. 163, pp. 185- 192 ,(1998) , 10.1111/J.1574-6968.1998.TB13044.X
Bobbie-Jo Kennedy, Laura A. Novotny, Joseph A. Jurcisek, Yves Lobet, Lauren O. Bakaletz, Passive transfer of antiserum specific for immunogens derived from a nontypeable Haemophilus influenzae adhesin and lipoprotein D prevents otitis media after heterologous challenge. Infection and Immunity. ,vol. 68, pp. 2756- 2765 ,(2000) , 10.1128/IAI.68.5.2756-2765.2000
Michael K Winson, Simon Swift, Philip J Hill, Catriona M Sims, Gottfried Griesmayr, Barrie W Bycroft, Paul Williams, Gordon S.A.B Stewart, Engineering the luxCDABE genes from Photorhabdus luminescens to provide a bioluminescent reporter for constitutive and promoter probe plasmids and mini-Tn5 constructs Fems Microbiology Letters. ,vol. 163, pp. 193- 202 ,(1998) , 10.1111/J.1574-6968.1998.TB13045.X
K Suzuki, L O Bakaletz, Synergistic effect of adenovirus type 1 and nontypeable Haemophilus influenzae in a chinchilla model of experimental otitis media. Infection and Immunity. ,vol. 62, pp. 1710- 1718 ,(1994) , 10.1128/IAI.62.5.1710-1718.1994
Annika Unge, Riccardo Tombolini, Lars Mølbak, Janet K. Jansson, Simultaneous Monitoring of Cell Number and Metabolic Activity of Specific Bacterial Populations with a Dualgfp-luxAB Marker System Applied and Environmental Microbiology. ,vol. 65, pp. 813- 821 ,(1999) , 10.1128/AEM.65.2.813-821.1999
Kevin M. Mason, Robert S. Munson, Lauren O. Bakaletz, A Mutation in the sap Operon Attenuates Survival of Nontypeable Haemophilus influenzae in a Chinchilla Model of Otitis Media Infection and Immunity. ,vol. 73, pp. 599- 608 ,(2005) , 10.1128/IAI.73.1.599-608.2005
Susan E.H. West, Mary Jo M. Romero, Laura B. Regassa, Nicolette A. Zielinski, Rodney A. Welch, Construction of Actinobacillus pleuropneumoniae-Escherichia coli shuttle vectors: expression of antibiotic-resistance genes. Gene. ,vol. 160, pp. 81- 86 ,(1995) , 10.1016/0378-1119(95)00236-Y
S. Timothy Motley, Brian J. Morrow, Xuejun Liu, Ingrid L. Dodge, Antonella Vitiello, Christine K. Ward, Karen Joy Shaw, Simultaneous analysis of host and pathogen interactions during an in vivo infection reveals local induction of host acute phase response proteins, a novel bacterial stress response, and evidence of a host-imposed metal ion limited environment. Cellular Microbiology. ,vol. 6, pp. 849- 865 ,(2004) , 10.1111/J.1462-5822.2004.00407.X
Kevin M. Mason, Robert S. Munson, Lauren O. Bakaletz, Nontypeable Haemophilus influenzae gene expression induced in vivo in a chinchilla model of otitis media. Infection and Immunity. ,vol. 71, pp. 3454- 3462 ,(2003) , 10.1128/IAI.71.6.3454-3462.2003
Laura A. Novotny, Guy Duquesne, Joe Cohen, Yves Lobet, Lauren O. Bakaletz, Bobbie-Jo Kennedy, Protection against development of otitis media induced by nontypeable Haemophilus influenzae by both active and passive immunization in a chinchilla model of virus-bacterium superinfection. Infection and Immunity. ,vol. 67, pp. 2746- 2762 ,(1999) , 10.1128/IAI.67.6.2746-2762.1999