Novel high-performance liquid chromatography for determination of membrane phospholipid composition of rat hepatocytes
作者： Yoshiaki Kurumi , Yukihiko Adachi , Tadashi Itoh , Hiroaki Kobayashi , Tatsuo Nanno
DOI: 10.1007/BF02781680
关键词:
摘要: We have developed a new, quick and efficient method of high-performance liquid chromatography (HPLC) for the isolation quantitative determination phospholipids in hepatocyte membranes. A silica gel column was used determination, an isocratic mixture acetonitrile, methanol 85% phosphoric acid (130:5:1.7, v/v/v) as mobile phase. Six kinds phospholipids, i.e. phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidylethanolamine (PE), phosphatidylcholine (PC), lysophosphatidylcholine (LPC) sphinogomyelin (SPH), this order, were completely isolated within 45 min. The phospholipid composition sinusoidal membrane vesicles (SMV) canalicular (CMV) obtained from rat liver, well human erythrocyte ghosts determined by HPLC method. level SPH CMV significantly higher than that SMV, PC lower SMV. These results considered attributable to low fluidity CMV. different SMV present technique is suitable cell
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sci-hub.se 参考文章(31)
M D Brown, P K Dudeja, T A Brasitus, S-adenosyl-L-methionine modulates Na+ + K+-ATPase activity in rat colonic basolateral membranes. Biochemical Journal. ,vol. 251, pp. 215- 222 ,(1988) , 10.1042/BJ2510215
M. Shinitzky, P. Henkart, Fluidity of Cell Membranes—Current Concepts and Trends International Review of Cytology. ,vol. 60, pp. 121- 147 ,(1979) , 10.1016/S0074-7696(08)61261-9
Meir Shinitzky, Yechezkel Barenholz, Dynamics of the Hydrocarbon Layer in Liposomes of Lecithin and Sphingomyelin Containing Dicetylphosphate Journal of Biological Chemistry. ,vol. 249, pp. 2652- 2657 ,(1974) , 10.1016/S0021-9258(19)42780-4
Otto A. Bessey, Oliver H. Lowry, Mary Jane Brock, A METHOD FOR THE RAPID DETERMINATION OF ALKALINE PHOSPHATASE WITH FIVE CUBIC MILLIMETERS OF SERUM Journal of Biological Chemistry. ,vol. 164, pp. 321- 329 ,(1946) , 10.1016/S0021-9258(18)43072-4
J L Farber, J T Martin, K R Chien, J Abrams, A Serroni, Accelerated phospholipid degradation and associated membrane dysfunction in irreversible, ischemic liver cell injury. Journal of Biological Chemistry. ,vol. 253, pp. 4809- 4817 ,(1978) , 10.1016/S0021-9258(17)30461-1
R.P. Van Hoeven, P. Emmelot, J.H. Krol, E.P.M. Oomen-Meulemans, Studies on plasma membranes. XXII. Fatty acid profiles of lipid classes in plasma membranes of rat and mouse livers and hepatomas. Biochimica et Biophysica Acta. ,vol. 380, pp. 1- 11 ,(1975) , 10.1016/0005-2760(75)90039-9
M Inoue, R Kinne, T Tran, L Biempica, I M Arias, Rat liver canalicular membrane vesicles. Isolation and topological characterization. Journal of Biological Chemistry. ,vol. 258, pp. 5183- 5188 ,(1983) , 10.1016/S0021-9258(18)32556-0
S.Shi-hua Chen, Anne Y. Kou, Improved procedure for the separation of phospholipids by high-performance liquid chromatography Journal of Chromatography B: Biomedical Sciences and Applications. ,vol. 227, pp. 25- 31 ,(1982) , 10.1016/S0378-4347(00)80352-7
A. Spinedi, S. Rufini, P. Luly, Age-dependent changes of rat liver plasma membrane composition Cellular and Molecular Life Sciences. ,vol. 41, pp. 1141- 1143 ,(1985) , 10.1007/BF01951698
Emmet B. Keeffe, Bruce F. Scharschmidt, Nancy M. Blankenship, Robert K. Ockner, Studies of Relationships among Bile Flow, Liver Plasma Membrane NaK-ATPase, and Membrane Microviscosity in the Rat Journal of Clinical Investigation. ,vol. 64, pp. 1590- 1598 ,(1979) , 10.1172/JCI109620