Improved procedure for the separation of phospholipids by high-performance liquid chromatography
作者： S.Shi-hua Chen , Anne Y. Kou
DOI: 10.1016/S0378-4347(00)80352-7
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摘要: Abstract We describe a rapid and efficient high-performance liquid chromatography procedure for the separation of phospholipids. The is accomplished on microparticulate silica gel column using isocratic elution UV detection at 203 nm. solvent mixture acetonitrile—methanol—85% phosphoric acid(130:5:1.5, v/v). Complete achieved within 30 min phosphatidylinositol, phosphatidylserine, phosphatidylethanolamine, phosphatidyldimethylethanolamine, lysophosphatidylethanolamine, phosphatidylcholine, lysophosphatidylcholine sphingomyelin. method suitable analysis phospholipids in tissue extracts.
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