A gamma-interferon-induced factor that binds the interferon response sequence of the MHC class I gene, H-2Kb.

摘要: Transcription of class I genes the major histocompatibility complex (MHC) can be induced by interferons. Treatment HeLa cells with interferon-gamma induces a DNA-binding factor, IBP-1, specific for site within interferon response sequence (IRS) H-2Kb promoter. The mol. wt as estimated photoactivated protein-DNA crosslinking analysis, is approximately 59 kd. Point-mutation this binding abolishes IBP-1 interaction and ability MHC promoter to respond interferon. Induction activity rapid closely parallels previously reported time course transcriptional activation endogenous genes. cycloheximide, protein synthesis inhibitor, blocked induction activity. An oligonucleotide derived from virus- double-stranded RNA-inducible interferon-beta 1 gene able bind IBP-1. Sequences similar are found upstream many interferon-responsive