Expression and distribution of fetuin in the developing sheep fetus.

摘要: Tissue distribution and developmental expression of fetuin were studied in the sheep fetus from embryonic day (E) 30 to adult (gestational period is 150 days). The presence was demonstrated immunocytochemically using anti-fetuin antibodies; situ hybridisation short anti-sense oligonucleotide probes labelled with digoxigenin used study ability developing tissue synthesise fetuin, reverse transcription-polymerase chain reaction (RT-PCR) estimate level mRNA selected tissues. widespread younger fetuses (E30 E40). most prominent due synthesis liver, central nervous system (CNS) including anterior horn cells, dorsal root ganglia skeletal muscle cells. Other tissues organs that showed evidence protein included mesenchyme, kidney, adrenal, bone, gut, lung heart. In immature liver (E30–40) there a strong signal for hepatocytes also numerous haemopoietic cells; proportion these latter cells positive increased between E30 E40. Only some stem immunoreactive itself at E30–40; more frequently observed mature outer regions liver. Lung gut contained scattered fetuin-positive epithelial especially E30; weak could be detected above background many up E40, but not E60–E115 or adult. Particularly mesenchymal both within such as around forming bone mRNA. Mesenchyme ages very strongly stained protein, much which may reflect extracellular spaces derived high concentration plasma. By E80 mainly present CNS; staining becoming less pronounced. However tissue, chondrocytes still older (E80) fetuses; growing surfaces bones marrow this age. adult, immunocytochemical hepatocytes, barely threshold limit detection. generally negative except precipitated plasma vessels their interstitial spaces. highest levels mRNA, by RT-PCR, E40 E60 followed muscle. low evident hybridisation, confirmed RT-PCR (about 0.1% E60). These results show immunocytochemically, its likely situ. instances (e.g. tissue) probably originates predominantly uptake fluid. functional significance different during development considered.