MiRNA-1225 Inhibits Osteosarcoma Tumor Growth and Progression by Targeting YWHAZ

摘要: Introduction Osteosarcoma is the most common bone tumor and characterized by presence of malignant mesenchymal cells produced in stroma. MiRNAs are known to function as post-transcriptional negative regulators gene expression. Emerging evidence showed that miR-1225-5P functions a suppressor several types cancers. The detailed mechanisms which suppresses growth not fully understood. objective present study was test hypothesis inhibits osteosarcoma cell vitro vivo targeting YWHAZ Methods Real-time PCR Western blot were carried out expression lines. Luciferase assay used demonstrate whether targets 3' UTR. To assess human lines, gain-of-function loss-of-function performed transfecting mimic or inhibitor into Furthermore, cycle analysis elucidate possible action cells. potential therapeutic effect miR-1225-5p tested xenograft mouse model, intravenous injection nude mice. Tumor sizes measured lung metastasis counted after mice sacrificed. Results inversely correlated with Database search revealed targeted Transfection downregulated expression, demonstrated real-time PCR, luciferase assay. Over-expression reduced growth, migration invasion downregulating Cell increased inhibiting inhibition rescued over-expression inhibited G1/G0 phase exit. In model metastasis. Conclusion Our findings suggested YWHAZ. This can serve method for treating osteosarcoma.