p53 exon 5 mutations as a prognostic indicator of shortened survival in non-small-cell lung cancer

摘要: Inactivation of the tumour-suppressor gene p53 has been described as one most common molecular changes found in lung tumours. Our purpose was to study prognostic value alterations and determine whether some specific mutation type could be associated with poor clinical evolution non-small-cell cancer (NSCLC) patients. To this end, we studied 81 resected primary NSCLCs order detect alterations. protein accumulation analysed using immunohistochemistry methods; mutations exons 5-9 were polymerase chain reaction-single-strand conformation polymorphism sequencing techniques. immunodetected 46.9% carcinomas 44.7% p53-immunopositive tumours showed mutations. Survival analysis performed on 62 No survival differences for patients or without immunopositivity. A shorter underlying mutations, mainly squamous cell tumours; worst prognosis when located exon 5 (P = 0.007). In conclusion, location might considered a indicator evaluation NSCLC