Detection by denaturant gradient gel electrophoresis of tumor-specific mutations in biopsies and relative bronchoalveolar lavage fluid from resectable non-small cell lung cancer.
作者： Ugo Pastorino , Giuseppe Curigliano , Lorenzo Spaggiari , Nicola Fazio , Giovanna Flamini
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摘要: A PCR-denaturant gradient gel electrophoresis (DGGE) method was developed for the detection of p53 and K-ras mutations in primary operable tumors paired BAL samples non-small cell lung cancer. Among 36 patients, 9 showed exon V biopsies three bronchoalveolar lavage (BAL) specimens with a 33% concordance. Five patients presented VI two BALs 40% No were found VII either or 100% Exon VIII six Of we detected 7 (19.4%) I on tumor samples. DGGE analysis DNA from revealed distributed 42% overall concordance respect to tissue. Molecular screening by p53-amplified had cumulative 46.6% sensitivity, specificity, 77.7% accuracy. 43% 88.8% test The proposed demonstrated be specific, accurate, at relatively low cost but limited sensitivity detecting presence neoplastic cells resectable
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