Transport of glycine by hemolyzed and restored pigeon red blood cells. Symmetry properties, trans effects of sodium ion and glycine, and their description by a single rate equation.

摘要: Abstract The aim of the present work was to devise a reasonably detailed transport model and derive an equation from it which would describe effects on unidirectional glycine substrates, Na+ glycine, simultaneously both sides membrane. is transition rather than static site model, passive in that energy for glycine-active comes solely gradient. Glycine entry exit kinetics, including dependence, were compared; influx kinetics under steady state equilibrium conditions inhibition by various external concentrations absence determined; relief this measured. With most measurements, six seven indispensable parameters evaluated as functions seventh. Expected values three remaining measurements could be calculated with since kinetic behavior insensitive seventh parameter, although individual rate constants strongly depend it. Agreement between predicted measured fair good. In addition general fit experiments several special properties found. system asymmetrical are not mirror images. asymmetry does arise active character transport. Formation complex containing more one must occur. Membrane-crossing empty, Na+-loaded, plus glycine-loaded mechanism different. That fully Na+-loaded form (ENa2) very much smaller other two, if zero. Transport hemolyzed restored cells similar those intact cells.