Induction of Cell Cycle Arrest and Apoptosis by Ruthenium Complex cis-(Dichloro)tetramineruthenium(III) Chloride in Human Lung Carcinoma Cells A549
作者： Aliny Pereira de Lima , Flávia de Castro Pereira , Cesar Augusto Sam Tiago Vilanova-Costa , Jordana Ribeiro Soares , Lucas Carlos Gomes Pereira
DOI: 10.1007/S12011-011-9275-7
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摘要: Lung cancer is one of the leading causes death in world, and non-small cell lung carcinoma (NSCLC) accounts for approximately 75-85% all cancers. In present work, we studied cytotoxic activity, cycle arrest induction apoptosis compound cis-(dichloro)tetramineruthenium(III) chloride {cis-[RuCl(2)(NH(3))(4)]Cl} human tumor line A549. The results MTT trypan blue assays showed that cis-[RuCl(2)(NH(3))(4)]Cl reduction viability A549 cells when treating with 95 383 μM 48 72 h. Lower concentrations (19, 3.8 0.38 μM), however, only slightly affected viability. IC(50) value was about μM. Survival analysis after treatment ruthenium(III) using long term clonogenic assay it reduced colony formation ability at μM, no colonies were observed. Cell ruthenium led to an accumulation S phase increased sub-G1 peak. addition, induced apoptosis, as observed by numbers annexin V-positive messenger RNA expression caspase-3.
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