Aptameric detection of quinine in reclaimed wastewater using a personal glucose meter

摘要: Quantification of small-molecule analytes in an aqueous environment remains expensive due to the high costs instruments and human resources. An aptamer–invertase biosensor coupled with a personal glucose meter (PGM) is precise promising method substitute traditional instruments. Herein, using quinine as example, we demonstrated feasibility quantify reclaimed wastewater. The analytical procedure consisted building structure aptamer–DNA–invertase on magnetic beads, competition between invertase, catalysis sucrose by released quantification PGM. dissociation coefficient aptamer was estimated be 0.36 μM isothermal titration calorimetry, indicating very strong affinity them. linear calibration curve range from 0 2 μM. limit detection (LOD) 0.13 for pure water 0.32 selectivity presence other pharmaceutics satisfactory. Thus, aptamer–invertase–PGM inexpensive, affordable feasible choice analysis wastewater management.