Homodirectional changes in transcriptome composition and mRNA translation induced by rapamycin and heat shock.

摘要: Transcription and mRNA turnover determine the quantitative composition of cellular transcriptome. The transcriptome in turn serves as a template for proteome via translation. Treatment Saccharomyces cerevisiae with TOR kinase inhibitor rapamycin causes increases decreases levels hundreds genes. We used DNA microarray analysis to monitor simultaneously translational changes all detectable yeast mRNAs. Notably, genes that are induced correlate tightly more efficiently translated mRNAs (based on their relative degree polyribosome association); similarly, show reduced after treatment also lower fitness. Microarray analyses heat-shocked cells reveal homodirectional co-regulatory responses. Thus, signal-induced amplified at level. These results unveil higher level coordinated gene regulation we refer 'potentiation.'