Sasanquasaponin protects rat cardiomyocytes against oxidative stress induced by anoxia-reoxygenation injury.

摘要: Reactive oxygen species can play an important role in the pathogenesis of anoxia-reoxygenation injury. Sasanquasaponin (SQS) is a biologically active ingredient extracted from Chinese medicinal plant Camellia oleifera Abel. Some studies have shown that SQS possesses potent antioxidant activities. However, it has not been elucidated whether diminishes reactive stress induced by injury cardiomyocytes. In this work, neonatal rat cardiomyocytes pretreated with test compound were subjected to anoxia-reoxygenation. The extent cellular damage was accessed cell viability and amount released lactate dehydrogenase (LDH). Superoxide dismutase, catalase glutathione peroxidase activities, reduced (GSH) oxidized (GSSG) levels, malondialdehyde contents measured colorimetric method. levels intracellular calcium determined flow cytometry. results showed LDH release increased dose-dependent manner up 10 μM concomitantly decreased GSSG contents, while significantly GSH activities superoxide peroxidase. Moreover, treatment alleviated accumulation undergoing It suggested could protect against oxidative attenuating generation increasing endogenous antioxidants.