CTLs contain and use intracellular stores of FasL distinct from cytolytic granules.

摘要: CTL lyse target cells through the release of cytolytic granule contents and cell surface expression Fas ligand (FasL). Current models suggest that FasL is stored in granules would be subject to same controls as degranulation. We demonstrate murine CTLs undergo two waves after stimulation. The first wave from a pre-existing pool FasL, second requires new protein synthesis. Signaling for appears finely tuned weak signal preferentially induced translocation whereas strong triggered de novo synthesized FasL. early differentially regulated degranulation, there were multiple circumstances whereby rapid FasL-dependent killing occurred absence detectable Furthermore, we found confocal microscopy resides vesicles distinct granules. Our data clearly show degranulation lytic mechanisms are fully independent with respect component localization regulation.