Hypoxia Regulates the Expression of PHLDA2 in Primary Term Human Trophoblasts
作者： H.-S. Kim , C.-R. Roh , B. Chen , B. Tycko , D.M. Nelson
DOI: 10.1016/J.PLACENTA.2006.01.025
关键词:
摘要: Abstract Hypoxia influences gene expression in placental trophoblasts. We sought to examine the effect of hypoxia on trophoblast human PHLDA2 (also termed IPL, TSSC3 or BWR-1C), a product an imprinted chromosome 11p15.5 whose murine ortholog plays pivotal role development. initially confirmed that was expressed term villi, primarily layer. Using quantitative PCR we found gradually declined during differentiation primary A similar pattern seen for p57 Kip2 and IGF-II, both products genes 11p15.5. Exposure trophoblasts vitro (O 2 ≤ 2%) markedly reduced mRNA protein. This not consistent among other products, as decreased, but IGF-II increased hypoxic exposed TGFβ1, -β2 -β3 unchanged. conclude down-regulates As limits growth, our results suggest down-regulation attenuates impact growth.
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sci-hub.se 参考文章(58)
John C. P. Kingdom, Peter Kaufmann, Oxygen and placental vascular development. Advances in Experimental Medicine and Biology. ,vol. 474, pp. 259- 275 ,(1999) , 10.1007/978-1-4615-4711-2_20
Andrew P. Feinberg, Maxwell P. Lee, Genomic Imprinting of a Human Apoptosis Gene Homologue, TSSC3 Cancer Research. ,vol. 58, pp. 1052- 1056 ,(1998)
Barry F. King, Gordon C Douglas, Differentiation of human trophoblast cells in vitro as revealed by immunocytochemical staining of desmoplakin and nuclei Journal of Cell Science. ,vol. 96, pp. 131- 141 ,(1990) , 10.1242/JCS.96.1.131
Vikram Budhraja, Edward Spitznagel, W Timothy Schaiff, Yoel Sadovsky, Incorporation of gene-specific variability improves expression analysis using high-density DNA microarrays BMC Biology. ,vol. 1, pp. 1- 8 ,(2003) , 10.1186/1741-7007-1-1
Karen D. Cowden Dahl, Benjamin H. Fryer, Fiona A. Mack, Veerle Compernolle, Emin Maltepe, David M. Adelman, Peter Carmeliet, M. Celeste Simon, Hypoxia-inducible factors 1α and 2α regulate trophoblast differentiation Molecular and Cellular Biology. ,vol. 25, pp. 10479- 10491 ,(2005) , 10.1128/MCB.25.23.10479-10491.2005
Sayeda N. Abu-Amero, Zehra Ali, Philip Bennett, Janet I. Vaughan, Gudrun E. Moore, Expression of the insulin-like growth factors and their receptors in term placentas: A comparison between normal and IUGR births Molecular Reproduction and Development. ,vol. 49, pp. 229- 235 ,(1998) , 10.1002/(SICI)1098-2795(199803)49:3<229::AID-MRD2>3.0.CO;2-Q
S. Rama, Y. Suresh, A.J. Rao, TGF β1 induces multiple independent signals to regulate human trophoblastic differentiation: Mechanistic insights Molecular and Cellular Endocrinology. ,vol. 206, pp. 123- 136 ,(2003) , 10.1016/S0303-7207(03)00202-8
M H Lee, I Reynisdottir, J Massague, Cloning of p57KIP2, a cyclin-dependent kinase inhibitor with unique domain structure and tissue distribution. Genes & Development. ,vol. 9, pp. 639- 649 ,(1995) , 10.1101/GAD.9.6.639
Tom Moore, David Haig, Genomic imprinting in mammalian development: a parental tug-of-war Trends in Genetics. ,vol. 7, pp. 45- 49 ,(1991) , 10.1016/0168-9525(91)90230-N
Jeh-Ping Liu, Julie Baker, Archlbald S. Perkins, Elizabeth J. Robertson, Argiris Efstratiadis, Mice carrying null mutations of the genes encoding insulin-like growth factor I (Igf-1) and type 1 IGF receptor (Igf1r) Cell. ,vol. 75, pp. 59- 72 ,(1993) , 10.1016/S0092-8674(05)80084-4