The Role of the Cucumber mosaic virus 2b Protein in Viral Movement and Symptom Induction

摘要: The Cucumber mosaic virus (CMV) 2b protein is a counter-defense factor and symptom determinant. Conserved domains in the sequence were mutated gene of strain Fny-CMV. effects these mutations assessed by infection Nicotiana tabacum, N. benthamiana, Arabidopsis thaliana (ecotype Col-0) with mutant viruses expression transgenes A. thaliana. We confirmed that two nuclear localization signals required for induction found N-terminal domain was essential induction. C-terminal serine residues within putative phosphorylation modulated severity. Further studies conducted using Fny-CMVΔ2b, cannot express induces no symptoms or ecotype Col-0. Surprisingly, plants C24, Fny-CMVΔ2b induced severe similar to those wild-type virus. However, C24 infected recovered from disease while did not. Expression either Fny-CMV LS-CMV (a mild strain) Col-0 enhanced systemic movement permitted Fny-CMVΔ2b. Taken together, results indicate itself an important determinant certain hosts. they also suggest may somehow synergize other CMV-encoded factors.