Detection and analysis of UV-induced mutations in mammalian cell DNA using a lambda phage shuttle vector

摘要: Abstract In order to study mutagenesis in mammalian cells, stable mouse L-cell lines were established with multiple copies of a lambda phage vector that contains the supF gene Escherichia coli as target for mutagenesis. Rescue viable from high molecular weight cell DNA using vitro packaging extracts was efficient (5 per microgram copy) and yielded negligible background mutant (0 out 54,605). From cells exposed 254-nm ultraviolet light (12J/m2), 78,510 rescued, which 8 found have genes. sequence analysis mutants suggests primary site UV is at pyrimidine-cytosine (Y-C) sequences, most frequent mutation this C----T transition.