Mechanism of action of salinomycin on growth and migration in pancreatic cancer cell lines.

摘要: Abstract Objectives Pancreatic cancer is one of the most aggressive and lethal cancers worldwide there are few effective treatments. Recently, salinomycin (Sal) was reported to alter proliferation apoptosis in various tumors. This prompted us investigate effect Sal on pancreatic cells explore possible molecular mechanism in vitro . Methods After treatment with Sal, cell viability were analyzed by Hoechst 33342 staining flow cytometry, respectively. Invasion metastasis assayed a Transwell migration assay. Flow cytometry also used assessed fraction CD133 + subpopulations. The expression proliferating nuclear antigen (PCNA), Bcl-2, E-cadherin, Wnt/β-catenin signaling-related proteins detected RT-PCR western blot. Results inhibited growth dose- time-dependent manner. We found that proportion subpopulations decreased after lines at same time. percentage apoptotic increased treatment. Compared control groups, Bax E-cadherin significantly upregulated, Bcl-2 PCNA downregulated Sal-treated cells. (β-catenin p-GSK-3β) inhibited. Conclusions These results indicate could influence , which may occur inhibition signaling.