An ELISA for detection of antibodies against influenza A nucleoprotein in humans and various animal species.
作者： G. F. de Boer , W. Back , A. D. M. E. Osterhaus
DOI: 10.1007/BF01310622
关键词:
摘要: A double antibody sandwich blocking ELISA, using a monoclonal (MAb) against influenza nucleoprotein (NP) was developed to detect antibodies influenza. Collections of serum samples were obtained from human and various animal species. All subtypes induced hemagglutinins NP. close correlation between titers the hemagglutination inhibition (HI) test NP-ELISA seen. Antibodies NP demonstrated in humans, ferrets, swine, horses, chickens, ducks, guinea pigs, mice, seals. The collected at intervals during prospective epidemiological studies, experimental natural infections, vaccination studies. decline maternal studied swine horses. enables rapid serological diagnosis is suited for screening, especially species which harbor several subtypes. HI neuraminidase tests, however, must still be used subtyping.
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