Enhanced gap junction formation with LDL and apolipoprotein B.
作者： Rita A Meyer , Paul D Lampe , Barbara Malewicz , Wolfgang J Baumann , Ross G Johnson
DOI: 10.1016/0014-4827(91)90457-6
关键词:
摘要: Abstract Gap junctions are plasma membrane specializations involved in direct cell-cell communication. Intercellular communication is dependent upon the assembly of gap junction structures and would be influenced by agents which alter process. We investigated effects low density lipoprotein (LDL) on between cultured Novikoff cells using quantitative dye transfer freeze-fracture electron microscopic methods. observed a concentration-dependent increase (maximum effect at 2.5 μg protein/ml) sixfold number aggregated particles per cell. Immunoblots probed with anti-connexin43 antibody revealed no detectable protein (connexin) levels. The influence different components LDL formation was also examined. First, we treated cholesterol (0–150 μ M ) serum-free BSA media decrease assembly. Second, added apolipoprotein-B (apo-B) phosphatidyl choline vesicles to fivefold addition without apo-B had formation. Thus, demonstrated that can modulated treatments.
elsevier.com
sci-hub.se 参考文章(51)
L L Kilgore, B W Patterson, W R Fisher, Immunologic comparison of the conformations of apolipoprotein B. Investigation of methodologies for the reconstitution of delipidated and denatured apolipoprotein B with nonionic surfactants. Journal of Biological Chemistry. ,vol. 261, pp. 8842- 8848 ,(1986) , 10.1016/S0021-9258(19)84458-7
A K Soutar, K Harders-Spengel, D P Wade, B L Knight, Detection and quantitation of low density lipoprotein (LDL) receptors in human liver by ligand blotting, immunoblotting, and radioimmunoassay. LDL receptor protein content is correlated with plasma LDL cholesterol concentration. Journal of Biological Chemistry. ,vol. 261, pp. 17127- 17133 ,(1986) , 10.1016/S0021-9258(19)76008-6
T Scott-Burden, T J Resink, A W A Hahn, U Baur, R J Box, F R Bühler, Induction of growth-related metabolism in human vascular smooth muscle cells by low density lipoprotein. Journal of Biological Chemistry. ,vol. 264, pp. 12582- 12589 ,(1989) , 10.1016/S0021-9258(18)63896-7
Rita A. Meyer, Jane Overton, Changes in intercellular junctions Developmental Biology. ,vol. 99, pp. 172- 180 ,(1983) , 10.1016/0012-1606(83)90264-6
L K Curtiss, R S Smith, Localization of two epitopes of apolipoprotein A-I that are exposed on human high density lipoproteins using monoclonal antibodies and synthetic peptides Journal of Biological Chemistry. ,vol. 263, pp. 13779- 13785 ,(1988) , 10.1016/S0021-9258(18)68310-3
S Lund-Katz, T L Innerarity, K S Arnold, L K Curtiss, M C Phillips, 13C NMR evidence that substitution of glutamine for arginine 3500 in familial defective apolipoprotein B-100 disrupts the conformation of the receptor-binding domain. Journal of Biological Chemistry. ,vol. 266, pp. 2701- 2704 ,(1991) , 10.1016/S0021-9258(18)49900-0
Alex B. Novikoff, A Transplantable Rat Liver Tumor Induced by 4-Dimethylaminoazobenzene Cancer Research. ,vol. 17, pp. 1010- 1027 ,(1957)
D.W. Laird, J.P. Revel, Biochemical and immunochemical analysis of the arrangement of connexin43 in rat heart gap junction membranes. Journal of Cell Science. ,vol. 97, pp. 109- 117 ,(1990)
D S Crow, E C Beyer, D L Paul, S S Kobe, A F Lau, Phosphorylation of connexin43 gap junction protein in uninfected and Rous sarcoma virus-transformed mammalian fibroblasts. Molecular and Cellular Biology. ,vol. 10, pp. 1754- 1763 ,(1990) , 10.1128/MCB.10.4.1754
J. L. Flagg-Newton, W. R. Loewenstein, Cell junction and cyclic AMP: II. Modulations of junctional membrane permeability, dependent on serum and cell density. The Journal of Membrane Biology. ,vol. 63, pp. 123- 131 ,(1981) , 10.1007/BF01969453