Describing the current status of Plasmodium falciparum population structure and drug resistance within mainland Tanzania using molecular inversion probes

摘要: High-throughput Plasmodium genomic data is increasingly useful in assessing prevalence of clinically important mutations and malaria transmission patterns. Understanding parasite diversity for identification specific human or populations that can be targeted by control programmes, to monitor the spread associated with drug resistance. An up-to-date understanding regional population dynamics also critical impact efforts. However, this largely absent from high-burden nations Africa, date, no such analysis has been conducted parasites Tanzania countrywide. To end, over 1,000 P. falciparum clinical isolates were collected 2017 13 sites seven administrative regions across Tanzania, genotyped at 1,800 variable positions genome-wide using molecular inversion probes. Population structure was detectable among Tanzanian parasites, approximately separating northern southern districts identifying genetically admixed north. Isolates nearby more likely related compared sampled distant districts. Known resistance seen increased frequency (including two infections carrying pfk13-R561H), additional variants undetermined significance antimalarial varied geography. Malaria Indicator Survey (2017) corresponded genetic findings, including average region-level complexity-of-infection estimates. The identified here provide information on extant spatial patterns which future surveys relatedness compared.