PfCG2, a Plasmodium falciparum protein peripherally associated with the parasitophorous vacuolar membrane, is expressed in the period of maximum hemoglobin uptake and digestion by trophozoites

摘要: A Plasmodium falciparum gene closely linked to the chloroquine resistance locus encodes PfCG2, a predicted 320-330kDa protein. In parasitized erythrocyte, PfCG2 expression rises sharply in trophozoite stage and is detected electron-dense patches along parasitophorous vacuolar membrane (PVM), cytoplasm digestive vacuole (DV). Results of extraction partitioning experiments show that peripheral Exposure trophozoite-infected erythrocytes trypsin-containing buffer after streptolysin O permeabilization indicates exposed erythrocyte cytosol at outer face PVM. highly susceptible hydrolysis by aspartic cysteine proteases shows dose-dependent accumulation presence protease inhibitors. These results suggest delivered from outside PVM DV, where it broken down parasite proteases. interacts with may be associated processes hemoglobin uptake digestion erythrocytic-stage parasites.