The importance of experimental time when assessing the effect of temperature on toxicity in poikilotherms.

摘要: Temperature is an important factor affecting toxicity, determining chemical toxicokinetics and toxicodynamics in poikilothermic organisms. Because metabolic rates are also affected by temperature, interactions between the emergence of toxic effects time very likely. The aim present study was to investigate how temperature affects toxicity copper toward nematode Caenorhabditis elegans when measured during short, fixed frames compared with full life cycles. Copper tested 2 experiments at 4 or 6 temperatures range 11 °C 24 °C, Cu concentrations spanning from 1 mg Cu/L agar 40 mg agar, respectively. Reproduction mortality were determined across entire cycle, production first egg population growth rate calculated. results showed that 50% effect (EC50s) increased 1.5-fold 2.5-fold increasing within range, depending on endpoint. When calculating EC50 reproduction after 24 h 96 h, typical setup for temperature-chemical interaction studies, ranged no much larger than those cycle. Studies must therefore be carefully designed relation research question being investigated. Environ Toxicol Chem 2014;33:1363–1371. © 2014 SETAC