Rat hepatic stellate cells contribute to the acute-phase response with increased expression of α1(I) and α1(IV) collagens, tissue inhibitor of metalloproteinase-1, and matrix-metalloproteinase-2 messenger RNAs☆

摘要: Abstract The acute-phase response (APR) represents a systemic reaction of the organism to multiple nonspecific inflammatory stimuli. In general, it is protective for host, and hepatocytes are main cells responding with alterations in expression set liver-specific proteins named proteins. We have previously shown that although turpentine-induced APR not fibrogenic per se, enhances collagen deposition rats treated CCl4 up-regulates hepatic α1(I) tissue inhibitor metalloproteinases 1 (TIMP-1) messenger RNAs (mRNAs). this report we extended our studies showed turpentine induced, time-dependent manner, α1(IV) collagens, TIMP-1, matrix-metalloproteinase 2 (MMP-2) mRNAs. further these mRNAs occurs stellate cells, but obtained 6 hours after induction an episode. These changes were accompanied by increased blood levels tumor necrosis factor α (TNF-α) interleukin (IL-6) without noticeable immediate their respective liver. contrast CCl4-induced liver damage, alone, whether administered as single dose or weekly 3 weeks did up-regulate transforming growth β1 (TGF-β1) mRNA result excess deposition. Overall, findings suggest livers repeated episodes may be enhanced only when given together stimulus activates (HSCs) and/or TGF-β1 expression. (H EPATOLOGY 2001;33:597-607.)