Plant-originated glycoprotein (36 kDa) suppresses interleukin-4 and -10 in bisphenol A-stimulated primary cultured mouse lymphocytes.

摘要: Bisphenol A (BPA) is one of the estrogen mimic environmental hormones and a chemical used for wrapping foods, toy products children, biomedical equipment, machines. It can exert toxic effects, such as occurring allergy-related diseases. This study demonstrates that glycoprotein isolated from Rhus verniciflua Stokes (RVS glycoprotein) has an inhibitory activity T-helper type 2 (Th2) cytokines [Interleukin (IL)-4 -10]. First, it was shown RVS inhibits proliferation lymphocytes scavenges intracellular reactive oxygen species (ROS). Then, activities mitogen-activated protein kinase (MAPK), GATA-binding protein-3 (GATA-3), t-box expressed in T-cells (T-bet), Th2 cell-related cytokine (IL-4 -10) were evaluated BPA (50 microM)-stimulated primary cultured mouse lymphocytes, using immunoblot analysis reverse-transcription polymerase chain reaction (RT-PCR). The results showed microg/mL) inhibited ROS, p38 MAPK dose dependently. In transcriptional factors oriented differentiation cells, significantly suppressed GATA-3, whereas enhanced T-bet. Also, (100 attenuated Th2-related -10). Taken together, obtained this suggest may help preventing immune dysfunction, produced by BPA.