Spectroscopic Studies of Drug-Nucleic Acid Interactions

摘要: At a preceding Jerusalem Symposium I had the pleasure of presenting initial results our development use oligonucleotides as models to study interaction drug-nucleic acid complexes (Krugh, 1974). The main thrust this early work was sequence preferences observed in actinomycin D with deoxydinucleotides studied by visible absorption spectroscopy 1972) and determination geometry formed using proton magnetic resonance (Krugh Neely 1973a,b). Several interesting developments have occurred understanding during past few years: some these will be discussed below. Figure 1 shows chemical structures (1a), ethidium bromide (1b), daunorubicin adriamycin (1c) actinomine (1d). Actinomycin D, are important drugs area cancer chemotherapy (e.g. see Meienhofer Atherton, 1973, Sartorelli, 1976, is trypanocidal drug LePecq et. al., 1974), while an analog which uncharged cyclic pentapeptides replaced positively charged (at neutral pH) groups shown 1d.