Tissue-specific expression of human angiotensin II AT1 and AT2 receptors and cellular localization of subtype mRNAs in adult human renal cortex using in situ hybridization.

摘要: All studies analyzing the localization of angiotensin II (Ang II) receptors in human kidney have been performed at protein level using 125I-Ang as a probe. In this study, cellular localizations Ang type l (AT1-R) and 2 (AT2-R) receptor mRNAs adult renal cortex were examined for first time situ hybridization, their expression patterns determined by RNase protection assay compared with those other tissues. obtained from tumor-free portions cell carcinoma, AT1-R mRNA levels about 8- to 10-fold higher than AT2-R levels. Human liver aorta predominantly expressed mRNA, while right atrium contained both mRNAs. Ligand-binding assays revealed that total number was 16.0 +/- 3.3 fmol/mg protein, similar (17.7 5. 8) but significantly (11.6 3.2) (5.6 2.7). Relative distribution ratios numbers 82/17 56/42%, respectively. hybridization study indicated strongest signals located interlobular arteries tubulointerstitial fibrous regions surrounding glomeruli, followed decreasing order glomeruli cortical tubules. Expression highly localized arteries. Cells present positive vimentin collagen 1, indicating majority cells are fibroblasts. Presence strong artery evidence, suggesting pharmacological framework differential effects subtype mediated function kidney.