Intrarenal localization of angiotensin II type 1 receptor mRNA in the rat

摘要: Intrarenal localization of angiotensin II type 1 receptor mRNA in the rat. We examined intrarenal (AT1) kidneys normal adult male Munich Wistar rats using methods reverse transcription-polymerase chain reaction (RT-PCR) and situ hybridization. For RT-PCR, we used a rat AT1 subtype A (AT1A)-specific oligonucleotide primer pair. To semiquantitatively assess expression level among several regions kidney, cDNA was coamplified with β-actin cDNA. When compared to adrenal gland (expressed as 100%), markedly higher glomeruli (273 ± 69%), followed intensity by renal papilla (151 57%), cortex (139 19%), medulla (114 35%). In hybridization studies, 479 bp nucleotide fragment from AT1A-coding exon probe, also revealed glomerular preponderant pattern localization. Thus, within glomerulus, localized mesangial areas, predominantly at vascular pole. components juxtaglomerular apparatus (JGA), namely terminal portion afferent arteriole (that is, immunohistochemically renin-positive site) extraglomerular cells, latter showed non-manipulated while undetectable outside JGA. The treated an I converting enzyme inhibitor (ACEI) extension on toward interlobular artery. speculate that observed localizing pole JGA may account for unique physiological pathophysiological actions (Ang II), is: (1) hemodynamic effect Ang involves simultaneous increases efferent arteriolar resistances reduction ultrafiltration coefficient; (2) experimental sclerosis, sensitive pharmacological inhibition II, is commonly prominent pole; (3) negative feedback relationship exists between renin, presumably via