Overexpression of sprouty 2 inhibits HGF/SF-mediated cell growth, invasion, migration, and cytokinesis.
作者： Chong-Chou Lee , Andrew J Putnam , Cindy K Miranti , Margaret Gustafson , Ling-Mei Wang
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摘要: A strict regulation of hepatocyte growth factor/scatter factor (HGF/SF)-Met signaling is essential for its appropriate function. Several negative regulators Met have been identified. Here we report that human Spry2 induced by HGF/SF and negatively regulates HGF/SF-Met signaling. We show overexpression inhibits cell proliferation, anchorage-independent growth, migration in wound-healing vitro invasion assays. Measured an electric cell-substrate impedance sensing biosensor, movement restricted, because dramatically facilitates attachment spreading enhancing focal adhesions increasing stress fibers. An analysis cycle distribution shows, unexpectedly, Spry2-GFP cells are polyploid. Thus, as with FGF EGF receptors, tempers downstream addition to pronounced effect on adhesion, it has properties suitable be considered a tumor-suppressor protein.
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