Viral aggregation: buffer effects in the aggregation of poliovirus and reovirus at low and high pH.

摘要: The effects of the buffer employed in maintaining a given pH value were tested on aggregation two viruses, poliovirus and reovirus. Poliovirus was found to aggregate at values 6 below, but not 7 or above, except borate buffer. Reovirus aggregated 4 only acetate tris(hydroxymethyl)aminomethane-citrate buffers 5. Other for reovirus 5 (succinate, citrate, phosphate-citrate) induced little aggregation. No significant above. For both most effective by having substantial monovalently charged anionic component, such as citrate 3. Cationic low pH, glycine, generally weaker aggregating ability than same pH. These results, when correlated with isoelectric point viruses (poliovirus 8.2; 3.9) indicated that strongly their overall charge positive, under certain circumstances negative. Although massively its point, remained dispersed point. conclusion can be drawn those conditions which one virus do necessarily induce it another.