Synergistic action of calcium ionophore A23187 and phorbol ester TPA on B-chronic lymphocytic leukemia cells.

摘要: The peripheral blood mononuclear cells from 16 patients with B-chronic lymphocytic leukemia (B-CLL, n = 13), B-prolymphocytic (B-PLL, 2) or hairy cell (n 1) were incubated in the presence of phorbol ester 12-0-tetradecanoylphorbol-13-acetate (TPA) and calcium ionophore A23187. A synergy between these inducers was found respect to morphological changes B proliferation differentiation. A23187 used alone did not activate cells. B-CLL treated double stimulus acquired a plasmacytoid morphology, showed significantly higher incorporation 3H-thymidine 3H-uridine, produced amounts monoclonal immunoglobulin compared same exposed either alone. These results indicate that act synergistically on induce B-PLL responded more vigorously signals provided by TPA Previous studies can mimic two physiological second messengers diacylglycerol inositol trisphosphate transduction leading activation, proliferation, differentiation normal present findings suggest capacity differentiate response messenger pathway is intact.