Classification of amyloidosis: misdiagnosing by way of incomplete immunohistochemistry and how to prevent it

作者: Reinhold P. Linke , Rosl Oos , Natalie M. Wiegel , Walter B.J. Nathrath

DOI: 10.1016/J.ACTHIS.2006.03.010

关键词:

摘要: Classification of every individual case amyloid disease is necessary in order to recognize its origin and possible pathogenesis for therapeutic consideration. the amyloids can be performed different ways. One method primarily exploits serum proteins-but these are risk factors only, therefore render only ancillary information. In principle, one cannot establish diagnosis alone through their use. Another approach analyzes deposited amyloids, either by extracting proteins followed immunochemical or chemical analysis, using immunohistochemistry. Based on analysis prototypes fibril proteins, we have developed a profile antibodies over years that specifically identify tissue sections. These been used as routine service clinicians pathologists immunohistochemically classifying found formalin-fixed The typing always controlled established classes. several cases, asked second opinion diagnosed class. Our own immunohistochemical data were then compared with those submitted. submitted results represented misdiagnoses classes most patients, since technique was usually incomplete. It purpose this report analyze such cases document some typical mistakes. Here, show how avoid common pitfalls arrive at correct immunohistochemistry appropriately.

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