Evidence of medium-chain-length polyhydroxyoctanoate accumulation in transgenic potato lines expressing the Pseudomonas oleovorans Pha-C1 polymerase in the cytoplasm
作者： Andrea Romano , Dick Vreugdenhil , Diaan Jamar , Linus H.W. van der Plas , Guy de Roo
DOI: 10.1016/S1369-703X(03)00030-5
关键词:
摘要: The phaC1 gene from Pseudomonas oleovorans, coding for the Pha-C1 polymerase, was introduced into potato genome. Transgenic callus and plant lines which transcribed translated transgene were selected cell suspension cultures wild type transgenic established. substrate 3-(R)-hydroxyoctanoate, provided to growth medium. In lines, but not in or without expression, evidence of medium-chain-length polyhydroxyalkanoate accumulation ranging 0.02 9.7 mg polymer per gram dry weight observed after feeding medium substrate.
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