Genomic sequencing method

作者: Randell L. Mills

DOI:

关键词:

摘要: A method of determining the nucleotide sequence a DNA molecule arbitrary length as single procedure by sequencing portions in fashion such that 5' end succeeding contiguous portion is sequenced 3' its preceeding sequenced, for all portions, where order determined molecule. Sequencing individual accomplished generating family polynucleotides under conditions which determine elements are partial copies and random on ends about dinucleotide an internal reference point; base composition terminal identity each element solving analysis wherein data used to solve assigning either or point entire built up from dinucleotide.

参考文章(5)
Maria Panico, Giovanni Sindona, Nicola Uccella, Bioorganic applications of mass spectrometry. 3. Fast-atom-bombardment-induced zwitterionic oligonucleotide quasimolecular ions sequenced by MS/MS Journal of the American Chemical Society. ,vol. 105, pp. 5607- 5610 ,(1983) , 10.1021/JA00355A013
Michael L. Gross, Philip A. Lyon, Asim Dasgupta, Naba K. Gupta, Mass spectral studies of probe pyrolysis products of intact oligoribonucleotides Nucleic Acids Research. ,vol. 5, pp. 2695- 2704 ,(1978) , 10.1093/NAR/5.8.2695