Quantitative Secretomic Analysis Identifies Extracellular Protein Factors That Modulate the Metastatic Phenotype of Non-Small Cell Lung Cancer

摘要: Lung cancer is the leading cause of cancer-related deaths for men and women in United States, with non-small cell lung (NSCLC) representing 85% all diagnoses. Late stage detection, metastatic disease lack actionable biomarkers contribute to high mortality rate. Proteins extracellular space are known be critically involved regulating every pathogenesis cancer. To investigate mechanism by which secreted proteins NSCLC, we performed quantitative secretomic analysis two isogenic NSCLC lines (NCI-H1993 NCI-H2073) an immortalized human bronchial epithelial line (HBEC3-KT) as control. H1993 was derived from a chemo-naive tumor, while H2073 primary tumor after etoposide/cisplatin therapy. From conditioned media these three lines, identified quantified 2713 proteins, including series inflammatory response, progr...