Conditional regulation of cyclooxygenase‐2 in tracheobronchial epithelial cells modulates pulmonary immunity

摘要: Cyclooxygenase-2 (COX-2) gene expression in the lung is induced pathological conditions such as asthma and pneumonia; however, exact impact of COX-2 airway regulating inflammatory immunological response not understood. To define a physiological role inducible epithelial cells, we developed novel line transgenic mice, referred to CycloOxygenase-2 TransActivated (COTA) that overexpress transgene distribution CC-10 promoter doxycycline. In doxycycline treatment, was increased epithelium COTA mice whole tissue contained three- sevenfold increase prostaglandin E(2) (PGE(2)), D(2) (PGD(2)) thromboxane B(2) (TXB(2)) 6-Keto F(2alpha) (PGF(2alpha)) compared wild-type untreated mice. Interestingly, primary mouse tracheal cells from produced only PGE(2) by doxycycline-induced activation, providing an indication cellular specificity terms mediator production. ovalbumin model, which given at sensitization stage, there interleukin (IL)-4 level addition, were treated with had impaired clearance Pseudomonas aeruginosa pneumonia has important determining infectious allergic agents.