Detection and molecular characterization of Giardia isolated from recreational lake water in Malaysia

作者: Yvonne A. L. Lim , Sharmila D. Ramasame , Mohammed A. K. Mahdy , Wan Yusoff W. Sulaiman , Huw V. Smith

DOI: 10.1007/S00436-009-1602-Y

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摘要: Nine 50-l surface water samples from a Malaysian recreational lake were examined microscopically using an immunomagnetisable separation-immunofluorescent method. No Cryptosporidium oocysts detected, but 77.8% of contained low numbers Giardia cysts (range, 0.17-1.1 cysts/l), which genetically characterised by SSU rRNA gene sequencing. Genotype analyses indicated the presence duodenalis assemblage A suggesting potential risk to public health. The present study represents first contribution our knowledge G. assemblages in water.

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