Expression and copy number analysis of TRPS1, EIF3S3 and MYC genes in breast and prostate cancer.
作者： K J Savinainen , M J Linja , O R Saramäki , T L J Tammela , G T G Chang
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摘要: The long arm of chromosome 8 is one the most common regions amplification in cancers several organs, especially carcinomas breast and prostate. TRPS1, MYC EIF3S3 genes are located minimal amplification, 8q23–q24, have been suggested to be target amplification. Here, our goal was study copy number expression three order investigate significance prostate cancer. By using fluorescence situ hybridisation (FISH), we first found that TRPS1 were amplified together about one-third hormone-refractory carcinomas. Next, analysed mRNA by real-time quantitative RT–PCR gene FISH six five cancer cell lines. Breast line, SK-Br-3, which contained highest all genes, showed overexpression only EIF3S3. Finally, levels measured freshly frozen clinical samples benign hyperplasia (BPH), as well untreated carcinoma. similar tumour groups, whereas higher (P=0.029) compared BPH. data suggest increased cancer, mechanisms underlying gene.
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F. M. Waldman, S. G. Hilsenbeck, C. K. Osborne, S. A. W. Fuqua, O.-P. Kallioniemi, J. J. Isola, L. W. Chu, Genetic aberrations detected by comparative genomic hybridization predict outcome in node-negative breast cancer American Journal of Pathology. ,vol. 147, pp. 905- 911 ,(1995)
Ritva Karhu, Jorma J. Isola, Olli-P. Kallioniemi, Anne H. Kallioniemi, Eija R. Hyytinen, Teuvo Tammela, Tapio Visakorpi, Ann-Christine Syvänen, Genetic Changes in Primary and Recurrent Prostate Cancer by Comparative Genomic Hybridization Cancer Research. ,vol. 55, pp. 342- 347 ,(1995)
Michael M. Lieber, David G. Bostwick, Junqi Qian, Robert B. Jenkins, Detection of c-myc oncogene amplification and chromosomal anomalies in metastatic prostatic carcinoma by fluorescence in situ hybridization. Cancer Research. ,vol. 57, pp. 524- 531 ,(1997)
Parastoo Momeni, Gernot Glöckner, Olaf Schmidt, Diane von Holtum, Beate Albrecht, Gabriele Gillessen-Kaesbach, Raoul Hennekam, Peter Meinecke, Bernhard Zabel, André Rosenthal, Bernhard Horsthemke, Hermann-Josef Lüdecke, Mutations in a new gene, encoding a zinc-finger protein, cause tricho-rhino-phalangeal syndrome type I. Nature Genetics. ,vol. 24, pp. 71- 74 ,(2000) , 10.1038/71717
Dominique Vidaud, Ivan Bièche, Rosette Lidereau, Michel Vidaud, Martine Olivi, Ingrid Laurendeau, Sengül Tozlu, Quantitation of MYC gene expression in sporadic breast tumors with a real-time reverse transcription-PCR assay Cancer Research. ,vol. 59, pp. 2759- 2765 ,(1999)
Michael L. Cher, Michael L. Cher, William B. Isaacs, G. Steven Bova, Sokhom S. Pin, Dan H. Moore, Peter R. Carroll, Eric J. Small, Jonathan I. Epstein, Ronald H. Jensen, Genetic alterations in untreated metastases and androgen-independent prostate cancer detected by comparative genomic hybridization and allelotyping Cancer Research. ,vol. 56, pp. 3091- 3102 ,(1996)
Katsura Asano, Hans-Peter Vornlocher, Nancy J. Richter-Cook, William C. Merrick, Alan G. Hinnebusch, John W. B. Hershey, Structure of cDNAs Encoding Human Eukaryotic Initiation Factor 3 Subunits POSSIBLE ROLES IN RNA BINDING AND MACROMOLECULAR ASSEMBLY Journal of Biological Chemistry. ,vol. 272, pp. 27042- 27052 ,(1997) , 10.1074/JBC.272.43.27042
Kimmo J. Savinainen, Outi R. Saramäki, Marika J. Linja, Ola Bratt, Teuvo L.J. Tammela, Jorma J. Isola, Tapio Visakorpi, Expression and gene copy number analysis of ERBB2 oncogene in prostate cancer. American Journal of Pathology. ,vol. 160, pp. 339- 345 ,(2002) , 10.1016/S0002-9440(10)64377-5
G. T. G. Chang, G.-J. C. M. van den Bemd, M. Jhamai, A. O. Brinkmann, Structure and function of GC79/TRPS1, a novel androgen-repressible apoptosis gene. Apoptosis. ,vol. 7, pp. 13- 21 ,(2002) , 10.1023/A:1013504710343
Bruce Anthony, Peggy Carter, Arrigo De Benedetti, Overexpression of the proto-oncogene/translation factor 4E in breast-carcinoma cell lines International Journal of Cancer. ,vol. 65, pp. 858- 863 ,(1996) , 10.1002/(SICI)1097-0215(19960315)65:6<858::AID-IJC25>3.0.CO;2-Z