Size-exclusion high-performance liquid chromatography of peptides. Requirement for peptide standards to monitor column performance and non-ideal behaviour.

摘要: A series of five synthetic peptide polymers with the sequence Ac-(G-L-G-A-K-G-A-G-V-G)n-amide, where n= 1–5, was employed to assess resolving power high-performance size-exclusion columns in separations. The standards showed great versatility monitoring both ideal (no interactions solutes column material) and non-ideal (hydrophobic and/or ionic behaviour volatile non-volatile mobile phases. effectiveness adding salts or organic solvents overcome non-specific materials well illustrated by standards. In addition, advantageous use highlighted. ability predict position elution order peptides during chromatography (SEC) requires be separated a pure process. Although demonstrated similar profiles non-denaturing medium on all studied this study suggested that, if conformational character peptideprotein mixture particular phase is uncertain required, SEC should carried out under highly denaturing conditions.