Quantitative 3-Dimensional Imaging of Murine Neointimal and Atherosclerotic Lesions by Optical Projection Tomography

摘要: OBJECTIVE Traditional methods for the analysis of vascular lesion formation are labour intensive to perform - restricting study 'snapshots' within each vessel. This was undertaken determine suitability optical projection tomographic (OPT) imaging 3-dimensional representation and quantification intimal lesions in mouse arteries. METHODS AND RESULTS Vascular injury induced by wire-insertion or ligation femoral artery administration an atherogenic diet apoE-deficient mice. Lesion examined OPT autofluorescent emission. Lesions could be clearly identified distinguished from underlying wall. Planimetric measurements area correlated well with those made histological sections subsequently produced same vessels (wire-injury: R²  =  0.92; ligation-injury: R²  =  0.89; atherosclerosis:  0.85), confirming both accuracy this methodology its non-destructive nature. It also possible record volumetric lumen these were highly reproducible between scans (coefficient variation   5.36%, 11.39% 4.79% wire- ligation-injury atherosclerosis, respectively). CONCLUSIONS These data demonstrate eminent atherosclerotic neointimal formation, providing a much needed means routine morphology studies type.