Epidermal Growth Factor Receptor Gene Expression in Estrogen Receptor-Positive and Negative Human Breast Cancer Cell Lines

摘要: Expression of epidermal growth factor (EGF) receptor by human breast cancer tissues has an inverse relationship with expression the estrogen and may be associated a poor clinical response. We have studied regulation EGF in series cell lines varying degrees responsiveness. Three receptor-positive lines, MCF-7, ZR-75-1, T47D, were found to less than 70,000 binding sites per radioreceptor assay stimulated vitro EGF. Four receptor-negative MDA-MB-231, Hs578T, EVSA-T, BT-20, contained greater showed no stimulation In all number was correlated amount protein RNA. Differences between types not due amplification gene. Rather, variations due, at least part, differences rate gene transcription as determined nuclear run-off studies. Our data confirm previously described receptors. show here that absence is higher levels functional mRNA.