Evidence for mobility of immunoglobulin domains obtained by spin-label method.
作者： A.I. Käiväräinen , R.S. Nezlin
DOI: 10.1016/0006-291X(76)90039-5
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摘要: Abstract It is found that EPR spectra of immunoglobulins and their subunits spin-labeled by iminoxyl radical 2,2,6,6-tetramethyl-4-amino (N-dichlorotriazine) at pH 7.5 are similar in form reflect the capability spin-label to be two states. Formation specific complexes antibodies with antigens accompanied increased correlation time labels as well fraction a more immobilized state. shown splitting light chains halves results label losing its capacity being rigid microenvironment. interpreted due relative motion domains.
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