Determination of erythromycin concentrations in rat plasma and liver by high-performance liquid chromatography with amperometric detection

摘要: Abstract A simple method for the quantitative determination of erythromycin (EM) concentrations in rat plasma and liver by high-performance liquid chromatography with amperometric detection was developed. EM extracted from 200 μl or homogenate sample under sodium hydroxide alkaline conditions tert.-butyl methyl ether. Oleandomycin used as an internal standard. The recovery rate up to 100%. detector cell potential oxidation +1100 mV. calibration curves were linear over concentration ranges 0.1–20.0 μg/ml 0.5–100.0 μg/g liver. applied rats after intravenous administration (50 mg/kg dose). presented here has proved be great use investigation pharmacokinetic characteristics small animals such rats.