Expression of Drug Resistance Proteins Pgp, MRP1, MRP3, MRP5 AND GST-π in Human Glioma

摘要: Chemotherapy in glioma is poorly effective: the blood–brain barrier and intrinsic and/or acquired drug resistance of tumor cells could partly explain this lack major effect. We investigated expression P-glycoprotein (Pgp), multidrug protein (MRP) 1, MRP3, MRP5 glutathione-S-transferase π (GST-π) malignant patients. Cytofluorimetric analysis 48 specimens 21 primary cultures showed high levels MRP1, moderate low Pgp, GST-π MRP3. Immunohistochemistry (25 specimens) (66.7% cases), MRP1 (51.3%), (45.8%), Pgp (34.8%) MRP3 (29.9%) cells. Moreover, samples by real time quantitative PCR mRNA all genes. Tumor vasculature, analyzed derived endothelial cells, proteins. Non-tumor brain (from a patient with arteriovenous malformation from one epilepsy), normal human astrocytes cultured were also analyzed: expressed highest proteins, mainly MRP5. No significant differences proteins detected between or recurrent gliomas, suggesting that chemoresistance mostly intrinsic. Therefore, we detected, for first time, presence on – both delineated an profile glioma. The possible association inhibitors efflux pumps chemotherapy be to improve drugs delivery into their cytotoxic effects.