Intermittent Reloading Attenuates Muscle Atrophy through Modulating Akt/mTOR Pathway

摘要: Purpose: The aim of this study is to investigate the effects intermittent reloading during hindlimb unloading (HU) on changes in intracellular signaling pathways skeletal muscle. Methods: Male Wister rats were divided randomly into one three experimental groups: 1) nonsuspended control, 2) HU for 7 d, and 3) with (HU/IR) 4 h[middle dot]d-1. After each period, antigravitational soleus muscle was analyzed. Results: d treatment, fiber atrophy (decrease relative mass: 0.28 mg[middle dot]g-1 group vs 0.36 control group, P < 0.05; decrease CSA: 1682.6 [mu]m2 2673.0 0.05) a phosphorylation levels anabolic pathway (Akt mTOR) observed. Additionally, expressions two types muscle-specific E3 ubiquitin ligase mRNA (muscle F-box (MAFbx), ring finger 1 (MuRF1)) upregulated atrophy. Increases binding activities nuclear factor kappa B (NF[kappa]B) also determined. In contrast, IR treatment attenuated (0.33 2067.5 [mu]m2) partially increased molecules. Expression MAFbx MuRF1 returned level, NF[kappa]B suppressed IR. Conclusion: These results suggest that IR-induced attenuation achieved by synergy between decreased catabolic mechanisms.